高效液相色谱法测定不同产地及品种灵芝三萜类成分的含量

Determination of triterpenoids in Ganodema lingzhi from different areas and species by HPLC

摘要:

【目的】建立同时测定灵芝子实体中灵芝酸C2、灵芝酸B、灵芝酸A、灵芝酮三醇、灵芝酸DM、灵芝酸T、灵芝醇B、灵芝酸S、灵芝酸G、灵芝酸F、灵芝酸D、灵芝稀酸B 12种三萜成分的高效液相色谱法。【方法】选用Aglient ZORBAX SB-Aq (4.6 mm×250 mm，5 μm)色谱柱，以乙腈-醋酸(0.01%)水溶液为流动相进行梯度洗脱，流速1.0 mL/min，分析波长252 nm，柱温30 °C，对不同产地及品种的灵芝样品进行测定分析。【结果】同一产地不同品种间三萜类成分含量具有显著差异，不同产地的灵芝样品三萜成分含量也有显著差异，所建方法精密度高，稳定性和重复性好。【结论】所建方法适用于12种三萜成分的同时测定。

Abstract:

[Objective] We established an HPLC (High Performance Liquid Chromatography) method to determine Ganoderic acid C2, Ganoderic acid B, Ganoderic acid A, Ganodermanontriol, Ganoderic acid DM, Ganoderic acid T, Ganoderiol B, Ganoderic acid S, Ganoderic acid G, Ganoderic acid F, Ganoderic acid D, Ganoderenic acid B in Ganodema lingzhi. [Methods] Aglient ZORBAX SB-Aq (4.6 mm×250 mm, 5 μm) was adopted as the chromatographic column, with acetonitrile-0.01% acetic acid solution using gradient elution at 30 oC with a flow rate of 1 mL/min, and the UV detection wavelength was set at 252 nm. Composition and content of different varieties of triterpenoids from different areas were determined by the above designed method. [Results] Triterpenoids in different varieties of Ganodema lingzhi in one area and samples from different areas were significantly varied in their compositions and contents; the method was sensitive, reliable and reproducible. [Conclusion] This method can be used to determine triterpenoids.