香蕉ADA1家族成员鉴定及其在生物和非生物胁迫下的表达分析

Identification of banana ADA1 gene family members and their expression profiles under biotic and abiotic stresses

摘要:

Spt-Ada-Gcn5-乙酰转移酶(Spt-Ada-Gcn5-acetyltransferase, SAGA)是高度保守的辅助转录起始复合物，转录接头蛋白-激活的改变/缺失亚基1 (alteration/deficiency in activation 1, ADA1)，也称作组蛋白H2A功能互作因子1 (histone H2A functional interactor 1, HFI1)，它是SAGA核心模块中的一个亚基，在植物的生长发育和抗逆性方面发挥着重要的作用。为了解香蕉ADA1的分子特性，本研究基于香蕉基因组数据库，对香蕉ADA1基因家族成员进行鉴定，分析其基本理化性质、系统进化、选择压力、启动子顺式作用元件及生物与非生物胁迫下的表达等。结果显示，香蕉A、B及阿宽蕉基因组中分别有10、6、7个ADA1家族成员；成员均为不稳定的亲水性蛋白，均保守地含有SAGA-Tad1结构域，MaADA1和MbADA1均可与SAGA核心模块中的SAGA相关因子11 (SAGA-associated factor 11, Sgf11)互作；系统发育显示香蕉ADA1基因家族成员可划分为3个亚族，进化过程中大多受纯化选择；香蕉ADA1基因家族成员的基因结构差异性较大；香蕉ADA1基因家族成员含有多个响应激素的作用元件；MaADA1-1可能对香蕉在低温胁迫下的抗性起着重要的作用，MaADA1均响应香蕉枯萎病菌胁迫。本研究表明，ADA1基因家族成员在香蕉中高度保守，并可能响应生物与非生物胁迫。

Abstract:

The Spt-Ada-Gcn5-acetyltransferase (SAGA) is an ancillary transcription initiation complex which is highly conserved. The ADA1 (alteration/deficiency in activation 1, also called histone H2A functional interactor 1, HFI1) is a subunit in the core module of the SAGA protein complex. ADA1 plays an important role in plant growth and development as well as stress resistance. In this paper, we performed genome-wide identification of banana ADA1 gene family members based on banana genomic data, and analyzed the basic physicochemical properties, evolutionary relationships, selection pressure, promoter cis-acting elements, and its expression profiles under biotic and abiotic stresses. The results showed that there were 10, 6, and 7 family members in Musa acuminata, Musa balbisiana and Musa itinerans. The members were all unstable and hydrophilic proteins, and only contained the conservative SAGA-Tad1 domain. Both MaADA1 and MbADA1 have interactive relationship with Sgf11 (SAGA-associated factor 11) of core module in SAGA. Phylogenetic analysis revealed that banana ADA1 gene family members could be divided into 3 classes. The evolution of ADA1 gene family members was mostly influenced by purifying selection. There were large differences among the gene structure of banana ADA1 gene family members. ADA1 gene family members contained plenty of hormonal elements. MaADA1-1 may play a prominent role in the resistance of banana to cold stress, while MaADA1 may respond to the Panama disease of banana. In conclusion, this study suggested ADA1 gene family members are highly conserved in banana, and may respond to biotic and abiotic stress.