草莓YABBY基因家族的鉴定及表达分析

Identification and expression analysis of the YABBY gene family in strawberry

摘要:

YABBY蛋白是一类调控植物形态建成与器官发育的重要转录因子。为了研究草莓YABBY，通过生物信息学技术鉴定了森林草莓(二倍体)与栽培草莓(八倍体)的YABBY基因家族成员及其序列特征，构建了各成员与植物YABBY的系统发育树与共线性关系图，并利用RNA-seq数据与定量逆转录聚合酶链反应(quantitative reverse transcription-polymerase chain reaction, qRT-PCR)技术分析其表达模式，构建绿色荧光蛋白(green fluorescent protein, GFP)融合表达载体，在烟草叶细胞中瞬时表达观察其亚细胞定位。研究结果表明，森林草莓有6个FvYABBY基因，栽培草莓有26个FxaYABBY基因；FvYABBY基因家族成员可归为5个不同的进化分支，与拟南芥AtYABBY基因家族存在5对直系同源。在森林草莓中，所有FvYABBY在根和花托中基本不表达，FvYABBY1、FvYABBY2、FvYABBY5和FvYABBY6在叶、茎、花和瘦果中高表达；在栽培草莓中，FxaYABBY1、FxaYABBY2、FxaYABBY5和FxaYABBY6在瘦果中表达，所有FxaYABBY在花托中为低表达或不表达。此外，在低温、高盐和干旱等非生物胁迫下，FvYABBY1、FvYABBY3、FvYABBY4和FvYABBY6下调表达，FvYABBY5上调表达，FvYABBY2则先上调后下调表达。在烟草叶细胞中，所有FvYABBY蛋白均定位在细胞核。以上结果为草莓YABBY基因的后续功能研究奠定了基础。

Abstract:

YABBY proteins are important transcription factors that regulate morphogenesis and organ development in plants. In order to study the YABBY of strawberry, bioinformatic technique were used to identify the YABBY gene families in Fragaria vesca (diploid) and Fragaria×ananassa (octoploid), and then analyze the sequence characters, phylogeny and collinearity of the family members. The RNA-seq data and the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) technique were used to assay the expression patterns of the family members. A green fluorescent protein (GFP) was fused with FvYABBYs and transiently expressed in tobacco leaf cells for the subcellular localization. As the results, six FvYABBY genes and 26 FxaYABBY genes were identified from F. vesca and F.×ananassa, respectively. The FvYABBY genes were grouped into five clades, and five family members were orthologous with AtYABBY genes of Arabidopsis. In F. vesca, all of the FvYABBYs were basically not expressed not expressed in root and receptacle, while FvYABBY1, FvYABBY2,FvYABBY5 and FvYABBY6 were highly expressed in leaf, shoot, flower and achene. In F.×ananassa, FxaYABBY1, FxaYABBY2, FxaYABBY5 and FxaYABBY6 were expressed in achene, and all FxaYABBY were poorly or not expressed in receptacle. Additionally, under the abiotic stresses of low temperature, high salt and drought, the expression of FvYABBY1, FvYABBY3, FvYABBY4 and FvYABBY6 were down-regulated, FvYABBY5 was up-regulated, and FvYABBY2 was up-regulated and then down-regulated. In tobacco leaf cells, the subcellular localization of FvYABBY proteins were in the nucleus. These results provides a foundation for the functional researches of YABBY gene in strawberry.