聚对苯二甲酸乙二醇酯水解酶检测方法的研究进展

Detection methods for polyethylene terephthalate degrading enzymes: a review

摘要:

聚对苯二甲酸乙二醇酯(polyethylene terephthalate,PET)是应用最广泛的合成聚酯之一。由于PET不易降解，在环境中积累，对陆地、水生生态系统以及人类健康构成严重威胁。基于生物酶催化的生物降解策略为PET回收利用提供了一种绿色途径，在过去20年间，已发现了多种PET水解酶，并通过蛋白质工程等手段来改善这些酶的降解性能，但是目前仍未找到适合大规模工业应用的PET水解酶。利用传统的检测方法筛选PET水解酶是一个缓慢而复杂的过程。为了促进PET酶法回收的工业化应用，需要研发高效的检测方法。近年来，研究人员开发了多种表征PET水解酶的分析方法。本文总结了可用于筛选PET水解酶的检测方法，如高效液相色谱法、紫外吸光度法和荧光激活液滴分选法等，并对其在筛选PET水解酶的应用方面进行了展望。

关键词:检测方法;聚对苯二甲酸乙二醇酯(PET)水解酶;荧光检测法;生物传感器

Abstract:

Polyethylene terephthalate (PET) is one of the most widely used synthetic polyester. It poses serious threat to terrestrial, aquatic ecosystems and human health since it is difficult to be broken down and deposited in the environment. The biodegradation based on enzymatic catalysis offers a sustainable method for recycling PET. A number of PET hydrolases have been discovered in the last 20 years, and protein engineering has increased their degradation capabilities. However, no PET hydrolases that are practical for widespread industrial use have been identified. Screening of PET hydrolase using conventional detection techniques is laborious and inefficient process. Effective detection techniques are required to promote the commercialization of PET hydrolases. Using efficient detection techniques to screen potent industrial enzymes is essential for supporting the widespread industrial implementation of PET hydrolases. To define PET hydrolase, scientists have created a number of analytical techniques recently. The detection techniques that can be used to screen PET hydrolase, including high performance liquid chromatography, ultraviolet absorption spectrometric, and fluorescence activated droplet sorting method, are summarized in this study along with their potential applications.

Key words:detection methods;polyethylene terephthalate (PET) hydrolase;fluorescence detection;biosensor