酶法生产L-高苯丙氨酸的研究进展

Advances in enzymatic production of L-homophenylalanine

摘要:

L-高苯丙氨酸(L-homophenylalanine,L-HPA)作为一种重要的非天然氨基酸，是合成治疗高血压的普利类药物等的关键中间体，具有广阔的市场前景。目前L-高苯丙氨酸的合成主要依赖于化学法，但化学合成L-高苯丙氨酸具有原料昂贵、步骤繁琐和污染严重等缺点，限制了广泛应用。因此，国内外研究者对L-高苯丙氨酸的酶法生产进行了深入的研究。本文就目前酶法合成L-高苯丙氨酸的工艺，包括脱氢酶法、转氨酶法、海因酶法和脱羧酶法的研究进展进行了综述，为酶法合成L-高苯丙氨酸提供一定的借鉴，为最终实现L-高苯丙氨酸的酶法工业化生产奠定基础。

Abstract:

L-homophenylalanine (L-HPA) is an important non-natural amino acid that has been used as a key intermediate for the synthesis of Puli drugs for the treatment of hypertension. At present, L-HPA is synthesized using chemical methods, which has the disadvantages of expensive raw materials, tedious steps and serious pollution. Therefore, researchers have conducted in-depth research on the enzymatic production of L-HPA. This review summarizes the research progress on the enzymatic synthesis of L-HPA, including the dehydrogenase process, the transaminase process, the hydantoinase process, and the decarboxylase process, with the hope to facilitate the industrial production of L-HPA.