沉默GmATG10导致大豆免疫反应的激活

Silencing GmATG10 results in activation of immune responses in soybean

摘要:

自噬途径是真核生物中普遍存在的物质降解及循环利用的保守机制，在真核生物的生长发育以及免疫反应等方面起着至关重要的作用。而ATG10在自噬体（autophagosomes）的形成过程中起着非常重要的作用。为探讨大豆（Glycine max） ATG10在免疫防御反应中的功能，本研究采用大豆豆荚斑驳病毒（bean pod mottle virus，BPMV）诱导的基因沉默技术（virus-induced gene silencing， VIGS）成功地在大豆中同时沉默ATG10的两个同源基因（GmATG10a和GmATG10b）；通过黑暗诱导的碳饥饿处理以及GmATG8积累水平的Western blotting分析证明，同时沉默GmATG10a/10b可导致大豆叶片出现自噬缺陷；抗病性鉴定与激酶分析证明沉默GmATG10a/10b可通过负调控GmMPK3/6激活而参与免疫反应，是大豆免疫反应的负调控因子。

Abstract:

Autophagy is a highly conserved mechanism for material degradation and recycling in eukaryote cells, and plays important roles in growth, development, stress tolerance and immune responses. ATG10 plays a key role in autophagosome formation. To understand the function of ATG10 in soybean, two homologous GmATG10 genes, namely GmATG10a and GmATG10b, were silenced simultaneously by bean pod mottle virus (BPMV) induced gene silencing. The carbon starvation induced by dark treatment and Western blotting analysis of GmATG8 accumulation level indicated that concurrent silencing GmATG10a/10b resulted in the impairment of autophagy in soybean; disease resistance and kinase assays demonstrated that GmATG10a/10b participated in the immune responses by negatively regulating the activation of GmMPK3/6, indicating that GmATG10a/10b plays a negative regulatory role in immune response in soybean.