绿豆VrWOX基因家族鉴定及表达分析

Molecular characterization and transcriptional analysis of VrWOX genes in mungbean[Vigna radiate (L.) Wilczek]

摘要:

WOX （WUSCHEL-related homebox）基因家族是植物特有的一类转录因子，是同源盒（homeobox，HB）转录因子超家族中的重要成员。WOX基因在植物干细胞调节及生殖发育过程中具有重要作用，其功能已在多个植物物种中鉴定。然而绿豆（Vigna radiate）VrWOX基因家族信息尚不清楚。本研究通过同源比对和聚类分析，在绿豆基因组中鉴定了42个VrWOX基因。VrWOX基因在绿豆染色体中分布不均，其中7号染色体含有的VrWOX数量最多。VrWOX基因分为古老进化支（19个VrWOX）、中等进化支（12个VrWOX）和年轻进化支（WUSCHEL进化支，11个VrWOX）3个亚类。种内和种间共线性分析发现，VrWOX基因共有12个重复事件，与拟南芥（Arabidopsis thaliana）AtWOX有15个同源基因对，与菜豆（Phaseolus vulgaris）PvWOX有22个同源基因对。VrWOX基因在基因结构、保守基序等方面存在很大差异，因而可能存在功能差异。VrWOX基因启动子区域含有不同种类和不同数量的顺式作用元件，导致VrWOX基因在不同组织中表现出不同的基因表达模式。本研究对VrWOX基因家族信息和表达模式进行了分析，为绿豆VrWOX基因功能和调控网络的解析奠定了一定的理论依据。

Abstract:

WUSCHEL-related homebox (WOX) gene family is a type of plant specific transcription factor, and belongs to the homeobox (HB) transcription factor superfamily. WOX genes play an important role in plant development, such as stem cell regulation and reproductive progress, and have been identified in many plant species. However, the information of mungbean VrWOX genes is limited. In this study, we identified 42 VrWOX genes in mungbean genome using Arabidopsis AtWOX genes as BLAST queries. VrWOX genes are unevenly distributed on 11 mungbean chromosomes, and chromosome 7 contains the most VrWOX genes. VrWOX genes are classified into three subgroups, the ancient group, the intermediate group and the modern/WUSCHEL group, which contains 19, 12 and 11 VrWOX members, respectively. Intraspecific synteny analysis revealed 12 VrWOX duplicated gene pairs in mungbean. Mungbean and Arabidopsis thaliana have 15 orthologous genes, and mungbean and Phaseolus vulgaris have 22 orthologous genes, respectively. The gene structure and conserved motif are different among VrWOX genes, indicating their functional diversity. The promoter regions of VrWOX genes contain different number and type of cis-acting elements, and VrWOX genes show distinct expression levels in eight mungbean tissues. Our study investigated the bioinformation and expression profiles of VrWOX genes, and provided essential information for further functional characterization of VrWOX genes.