中国仓鼠卵巢细胞表观遗传调控研究进展

Advances in epigenetic regulation of Chinese hamster ovary cells

摘要:

中国仓鼠卵巢(Chinese hamster ovary, CHO)细胞因其具有可悬浮培养及进行蛋白质糖基化等翻译后修饰等优势，在生物制药重组蛋白生产方面具有不可替代的重要作用。但转基因沉默、表观遗传修饰等影响基因表达调控，造成CHO细胞表达稳定性降低而导致重组蛋白产量下降。本文对CHO细胞中表观遗传修饰包括DNA甲基化、组蛋白修饰和miRNA的作用研究，以及对基因表达调控的影响进行了综述。

关键词:中国仓鼠卵巢细胞;表观遗传调控;DNA甲基化;组蛋白修饰;microRNA(miRNA)

Abstract:

Chinese hamster ovary (CHO) cells play an irreplaceable role in biopharmaceuticals because the cells can be adapted to grow in suspension cultures and are capable of producing high quality biologics exhibiting human-like post-translational modifications. However, gene expression regulation such as transgene silencing and epigenetic modifications may reduce the recombinant protein production due to the decrease of expression stability of CHO cells. This paper summarized the role of epigenetic modifications in CHO cells, including DNA methylation, histone modification and miRNA, as well as their effects on gene expression regulation.

Key words:Chinese hamster ovary (CHO) cells;epigenetic regulation;DNA methylation;histone modification;microRNA (miRNA)