植物代谢组学中几种重要的次生代谢物液质分析技术研究进展

Advances in liquid chromatography-mass spectrometry analysis of several important secondary metabolites in plant metabolomics

摘要:

代谢组学（metabolomics）主要是研究生物体、组织、细胞的代谢物组分及检测其动态变化过程，是继基因组和蛋白组学后新兴的一门组学技术。代谢物是细胞调节过程中的最终产物，其水平被视为生物系统对遗传或环境变化的最终反映。通过合适的分析平台，准确定性、定量在复杂的生物中具有化学多样性的次生代谢物是代谢组学的一项重要工作。液相色谱-串联质谱技术（liquid chromatography-tandem mass spectrometry，LC-MS/MS）是代谢物质检测平台最常用的方法，也为植物次生代谢物的广泛应用研究提供了基础。本文主要从植物激素类、叶酸类、黄酮类等次生代谢物方面进行阐述，结合液质联用技术，简要论述不同次生代谢物检测技术的研究进展。

Abstract:

Metabolomics, which mainly studies the metabolite components of organisms, tissues, cells and their dynamic changes, is an emerging omics technology following genomics and proteomics. Metabolites are the final products of cellular regulation, and the concentration of metabolites is considered to be the ultimate response of a biological system to genetic or environmental changes. Secondary metabolites with chemical diversity are widely present in living organisms, thus accurate quantification of secondary metabolites through appropriate analytical platforms is an important task of metabolomics. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the most commonly used method for the detection of metabolites, providing a basis for the wide application of plant secondary metabolites. This review summarizes the advances of using LC-MS/MS techniques for the detection of phytohormone, folic acid, flavonoids and other secondary metabolites.