胶体金免疫层析试纸条技术在病毒检测领域的应用研究现状
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江苏省农业科技自主创新资金项目(CX(21)3162);国家自然科学基金青年项目(32002420)


Colloidal gold immunochromatographic test strip for virus detection:a review
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    摘要:

    胶体金免疫层析试纸条技术是一种快速、灵敏和精准的固相标记检测技术,胶体金免疫层析试纸条具有价格低廉、操作简便、检测快捷和特异性强的优点,具有在短时间内灵敏、准确地定性检测出相关病毒的潜在能力,有效解决传统检测方法在医学、兽医、动植物病毒检测和农药残留检测等领域存在检测时间长、设备不便和专业性强的弊端。目前在检测领域,该技术在检测细菌性疾病、病毒性疾病和预防传染性疾病大面积扩散等方面都有应用,因此,该技术在检验方面具有巨大的发展空间。文中主要对胶体金免疫层析技术进行综述,并对该技术在生物病毒检测方面进行总结和展望。

    Abstract:

    Colloidal gold immunochromatographic strip is a fast, sensitive and accurate solid-phase labeling detection technology, which has the advantages of low price, easy operation, rapid detection and high specificity, with the potential to qualitatively detect the relevant viruses in a short time with desired sensitivity and accuracy. It effectively addresses the disadvantages of long detection time, equipment inconvenience and professionalism requirement of the traditional detection methods used in the medical, veterinary, animal, plant virus detection, pesticide residue detection and other areas. Presently, the technology has been applied in the detection of bacterial diseases, viral diseases and prevention of extensive spread of infectious diseases, and has sufficient room for further development. This review summarizes the application of colloidal gold immunochromatography strip for biological virus detection, followed by prospecting future perspectives.

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董旭旭,孙威,曹攀,刘晓丹. 胶体金免疫层析试纸条技术在病毒检测领域的应用研究现状[J]. 生物工程学报, 2022, 38(9): 3243-3254

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  • 收稿日期:2022-01-21
  • 录用日期:2022-03-21
  • 在线发布日期: 2022-09-24
  • 出版日期: 2022-09-25
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