基于双酶级联协调表达策略高效催化合成D-甘露醇

Efficient biosynthesis of D-mannitol by coordinated expression of a two-enzyme cascade

摘要:

D-甘露醇(D-mannitol)作为合成抗肿瘤药和免疫刺激剂的重要前体被广泛应用于制药和医疗等行业,酶法合成D-甘露醇反应成本昂贵无法满足工业化生产。本研究首先筛选关键酶获得较优性能的甘露醇脱氢酶LpMDH和用于辅因子NADH再生的葡萄糖脱氢酶BaGDH,在大肠杆菌(Escherichia coli) BL21(DE3)中共表达,实现了基于双酶级联反应催化底物D-果糖合成D-甘露醇,D-甘露醇的初步摩尔转化率为59.7%。针对双酶级联催化反应中辅酶再生用酶与催化用酶表达量不协调的问题,通过增加Bagdh拷贝量来提高辅因子循环能力,获得了双酶催化速率平衡的重组大肠杆菌E.coli BL21/pETDuet-Lpmdh-Bagdh-Bagdh。进一步对重组菌的全细胞转化条件进行优化,确定了最适转化条件为反应温度30℃,初始pH值6.5,菌体量OD₆₀₀=30,底物D-果糖100.0 g/L,辅底物葡萄糖与底物1︰1摩尔当量。于最优转化条件下5 L发酵罐转化24 h,D-甘露醇的最高产量为81.9g/L,摩尔转化率为81.9%。本研究提供了一种绿色、高效生物催化生产D-甘露醇的方法,为实现其规模化生产奠定了基础,同时也对其他相关稀有糖醇的研究具有指导意义。

Abstract:

D-mannitol is widely used in the pharmaceutical and medical industries as an important precursor of antitumor drugs and immune stimulants. However, the cost of the current enzymatic process for D-mannitol synthesis is high, thus not suitable for commercialization. To address this issue, an efficient mannitol dehydrogenase LpGDH used for the conversion and a glucose dehydrogenase BaGDH used for NADH regeneration were screened, respectively. These two enzymes were co-expressed in Escherichia coli BL21(DE3) to construct a two-enzyme cascade catalytic reaction for the efficient synthesis of D-mannitol, with a conversion rate of 59.7% from D-fructose achieved. The regeneration of cofactor NADH was enhanced by increasing the copy number of Bagdh, and a recombinant strain E. coli BL21/pETDuet-Lpmdh-Bagdh-Bagdh was constructed to address the imbalance between cofactor amount and key enzyme expression level in the two-enzyme cascade catalytic reaction. An optimized whole cell transformation process was conducted under 30 ℃, initial pH 6.5, cell mass(OD₆₀₀) 30,100 g/L D-fructose substrate and an equivalent molar concentration of glucose. The highest yield of D-mannitol was 81.9 g/L with a molar conversion rate of 81.9% in 5 L fermenter under the optimal conversion conditions. This study provides a green and efficient biotransformation method for future large-scale production of D-mannitol, which is also of great importance for the production of other sugar alcohols.