高效液相色谱法测定生物脱硫系统中的含硫化合物

Determination of sulfur compounds in biological desulfurization system by high performance liquid chromatography

摘要:

生物脱硫是利用微生物脱除气体和石油中的含硫化合物，具有操作条件温和、工艺流程简单、脱硫效率高、能量消耗低和环境污染少等优点。但是，当前仍然缺乏简单高效的分析方法来定量分析生物脱硫过程中的含硫化合物。针对这个问题，建立了柱前荧光衍生高效液相色谱法同时测定生物脱硫溶液中的亚硫酸盐、硫代硫酸盐和硫化物的分析方法。该分析方法中含硫化合物的标准曲线具有良好的线性关系，亚硫酸盐、硫代硫酸盐和硫化物相关系数分别为0.999 46、0.999 67和0.999 65，其检测限分别为0.000 6 μmol/L、0.000 7 μmol/L和0.001 1 μmol/L；含硫化合物的加标回收率范围分别为98.17%–101.92%、100.90%–102.60%和101.11%–104.22%；并具有良好的重复性和稳定性。实验证明，该分析方法预处理简单、分析快速、结果准确，可用于同时测定不同生物脱硫系统中的含硫化合物。

Abstract:

Biological desulfurization is a process in which sulfur compounds are removed from gas and oil using microorganisms. It is a simple process that has mild operating conditions, high desulfurization efficiency, low energy consumption and less environmental pollution. However, there is still a lack of simple and efficient analytical methods for quantitatively analyzing the sulfur compounds in the biological desulfurization process. In order to solve this problem, the analytical method for the simultaneous determination of sulfite, thiosulfate and sulfide in biological desulfurization solutions by pre-column fluorescence derivation using high performance liquid chromatography (HPLC) was developed. The standard curves of sulfur species in this analytical method had good linear relationships with correlation coefficients of 0.999 5, 0.999 7, and 0.999 7 for sulfite, thiosulfate and sulfide, respectively. The detection limits of these sulfur compounds were 0.000 6, 0.000 7 and 0.001 1 μmol/L; the range of recovery rates were 98.17 to 101.9%, 100.9 to 102.6%, and 101.1 to 104.2%; which had good repeatability and stability. The analytical method was simple, efficient and accurate, and could be used to simultaneously determine the sulfur compounds in different biological desulfurization systems.