微生物微培养系统研究现状与展望
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国家重大科学仪器设备开发专项 (No. 21627812),“十三五”国家重点研发计划 (No. 2016YFF0202303),清华大学自主科研项目 (No. 20161080108) 资助。


Micro-cultivation system in microbiology: frontiers and prospects
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National Key Scientific Instrument and Equipment Project of The National Natural Science Foundation of China (No. 21627812), National Key Research and Development Program of China (No. 2016YFF0202303), Tsinghua University Initiative Scientific Research Program (No. 20161080108).

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    摘要:

    微生物培养是微生物学的起源和根本,成功的微生物培养是进行后续研究的重要前提与基础。但是,微生物培养操作不仅耗费了科研人员大量的精力和时间,还存在着极大的人为误差。近年来,容量小、高通量、模块化、可操作性良好并可进行在线检测的微生物微培养系统得到了微生物学领域的广泛关注。文中介绍了应用于微生物学领域的微培养系统,并按系统构成分类讨论了微生物微培养系统的发展、应用和展望。

    Abstract:

    Microbial cells cultivation is not only the origin, but also the foundation of microbiology. Researches in microbiology can only be carried out when the microbial cells can be cultured. However, conventional microbial cell cultivation is not only time consuming and labour intensive, but human error is also inevitable. Recent years, automated, modularised microbial cells micro-cultivation systems with small volume, good controllability, and equipped with real-time monitoring system have attracted great attention in microbiology. This review presents the state-of-the-art micro-cultivation systems which are implemented in microbial cells cultivation. The key development, applications of various system classified based on their construction, and the prospects of micro-cultivation system are discussed and insights into them are also provided.

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陈政霖,马春玄,邢新会,张翀. 微生物微培养系统研究现状与展望[J]. 生物工程学报, 2019, 35(7): 1151-1161

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  • 收稿日期:2018-03-13
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  • 在线发布日期: 2019-07-19
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