CRISPR-Cas9基因编辑技术在秀丽线虫中的应用

CRISPR-Cas9 mediated genome editing in Caenorhabditis elegans

摘要:

基于细菌基因组规律成蔟的间隔短回文重复 (Clustered regularly interspaced short palindromic repeats)发展而来的新型基因编辑方法 (CRISPR-Cas9) 对生物医学研究是一场划时代的革命。它几乎可用于大多数生物体的基因编辑。秀丽线虫是一种非常经典的遗传学模式生物，CRISPR-Cas9基因编辑技术进一步加速了对其基因功能及各种生物学问题的研究。文中主要总结CRISPR-Cas9基因编辑系统在遗传学模式生物秀丽线虫中的发展和应用。

Abstract:

The development of genome editing techniques based on CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system has revolutionized biomedical researches. It can be utilized to edit genome sequence in almost any organisms including Caenorhabditis elegans, one of the most convenient and classic genetic model animal. The application of CRISPR-Cas9 mediated genome editing in C. elegans promotes the functional analysis of gene and protein under many physiological conditions. In this mini-review, we summarize the development of CRISPR-Cas9-based genome editing in C. elegans.