鸡腿菇子实体多糖的分离纯化、理化性质及抗氧化活性

Separation, purification and antioxidant activity of polysaccharide from Coprinus comatus

摘要:

本研究对水溶醇沉法提取的鸡腿菇粗多糖脱蛋白方法进行了比较，最终确定Sevage法为最优的脱蛋白方法。经DEAE-纤维素52离子交换及Sephadex G-200分子层析柱分级、纯化，最终得到2个主要的多糖组分Ccp-I-A和Ccp-I-B。理化性质测定结果表明，二者均为白色絮状固体，能溶于水，不溶于无水乙醇、丙酮等有机溶剂；与斐林试剂，CTAB、硫酸-咔唑、碘-碘化钾及三氯化铁反应均为阴性。GC测定结果可知Ccp-I-A主要由甘露糖、葡萄糖和半乳糖组成，摩尔比为2.03∶9.52∶1；Ccp-I-B主要由岩藻糖和半乳糖组成，其摩尔比为：1∶5.21。另外，Ccp-I-A和Ccp-I-B对DPPH和·OH显示出良好的清除能力，而且，相比于Ccp-I-B，Ccp-I-A的清除能力更高，当浓度为300 μg/mL，其对DPPH和·OH的清除能力可分别达到72.1%和55.3%。

Abstract:

We compared the ways of deproteinization for crude polysaccharides of Coprinus comatus, and finally selected Sevage method as the optimal method. Two main fractions of Ccp-I-A and Ccp-I-B were obtained after DEAE-52 cellulose and Sephadex G-200 chromatography, both were white-floc, soluble in water, insoluble in absolute ethyl alcohol acetone and other organic solvents. Additionally, Fehling reagent, CTAB, Sulphuric acid-carbazole, iodine-potassium iodide reaction and ferric trichloride reaction were all negative. GC analysis showed Ccp-I-A was composed of mannitose, glucose and galactose in molar ratios of 2.03:9.52:1, whereas Ccp-I-B was composed of fucose and galactose with molar ratios of 1:5.21. Antioxidant activity test showed that Ccp-I-A and Ccp-I-B had good scavenging abilities on DPPH and ·OH. Compared to Ccp-I-B,the scavenging activity of Ccp-I-A was much stronger (72.1% versus 55.3%) when the concentration was 300 μg/mL.