IFIT家族基因抑制A型流感病毒WSN毒株的复制和转录

Inhibition of replication and transcription of WSN influenza A virus by IFIT family gene

摘要:

IFIT (Interferon induced proteins with tetratricopeptide repeats) 家族基因是一组较早发现的干扰素刺激基因，它在抗病毒和免疫调节中发挥了重要作用。为研究IFIT家族基因抑制A型流感病毒复制的机理，利用高通量RNA深度测序 (RNA-Seq) 技术发现A型流感病毒A/WSN/33 (WSN) 毒株感染293T细胞后，IFIT家族基因均出现明显上调。同时发现在IFIT2、IFIT3过表达后，流感病毒的复制和转录均有明显下调，并对vRNP聚合酶活性具有剂量依赖型的抑制作用。进一步研究证明在感染IFIT2、IFIT3编码蛋白与流感病毒非结构蛋白 (NS1) 存在细胞内共定位，证明二者存在相互作用的可能。综上所述，IFIT家族基因可以抑制A型流感病毒的复制和转录，有助于进一步阐明宿主因子对流感病毒感染的调节机制。

Abstract:

IFIT family genes are a kind of interferon stimulated genes (ISGs), and play important roles in antiviral sector and immunity regulation. To study the regulatory effect of IFIT family genes during influenza A virus (IAV) infection, we used RNA-sequencing analysis (RNA-Seq) technique and found that when 293T cells were infected by A/WSN/33 (WSN), the concentration of IFIT family genes were increased. Further study reveals that overexpression of IFIT2 or IFIT3 could inhibit IAV replication and transcription, and cause the dose-dependent inhibition of polymerase activity of vRNP. In addition, IFIT2 and IFIT3 encoding protein could colocalize with NS1 in 293T cells infected by WSN, indicating that they might interact with each other. The results suggest that IFIT family genes can inhibit the replication and transcription of IAV, which contributes to our understanding of the regulatory effect of host factors during influenza virus infection.