高精度相对和绝对定量的等量异位标签在定量蛋白质组学研究中的新进展

Recent advance in high accuracy iTRAQ for quantitative proteomics

摘要:

蛋白质组学逐渐从定性研究转向定量研究。在定量蛋白质组学技术中，相对和绝对定量的等量异位标签 (Isobaric tags for relative and absolute quantitation, iTRAQ) 是应用最广泛的技术之一，具有通量高、稳定性强及不受样品来源制约等优点，几乎可以对任意样品进行标记，而且可以同时对多达8个样品进行定量分析，有效地提高了通量。iTRAQ技术不断改进，其定量准确性显著提高，适用的平台越来越多，为微生物、动物、植物、生物医学领域蛋白质及其翻译后修饰组研究创造了条件。文中综述了高精度iTRAQ技术在定量蛋白质组学研究中的最新发展及其应用。

Abstract:

Nowadays, proteomics focuses on quantitative analysis rather than qualitative. In the field of quantitative proteomics, Isobaric tags for relative and absolute quantitation (iTRAQ) is one of the most widely used techniques. The advantage of iTRAQ is high throughput, high stability and free of the restriction of sample property. iTRAQ is suitable for almost all kinds of samples, and up to 8 samples can be analyzed simultaneously by commercially available kit. Along with the development of techniques, more and more mass spectrometry (MS) platforms are used in iTRAQ experiments and the accuracy of iTRAQ has been improved. iTRAQ has been applied to studies of microorganism, animal, plant, medical and protein post-translational modification. Here we review the recent progress in the development of iTRAQ and its applications in quantitative proteomics.