响应面优化产琥珀酸放线杆菌生产丁二酸

Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology

摘要:

丁二酸是一种重要的C4化合物平台，可以合成一系列重要化合物。文中对产琥珀酸放线杆菌Actinobacillus succinogenes GXAS137发酵生产丁二酸培养基成分进行优化。通过单因素和Plackett-Burman试验设计筛选出影响丁二酸发酵的重要参数，采用最陡爬坡实验逼近最大丁二酸生产区域后，利用Box-Behnken设计确定重要参数的最佳水平。筛选结果表明，影响丁二酸产量的重要参数是葡萄糖、酵母提取物和碱式碳酸镁浓度。最佳条件为(g/L)：葡萄糖70.00，酵母提取物9.20，碱式碳酸镁58.10。优化后丁二酸产量达到47.64 g/L。与初始条件 (36.89 g/L) 相比，丁二酸浓度提高了30 %。在最佳工艺条件下得到的试验结果与模型预测值很吻合，说明建立的模型是有效的。

Abstract:

Succinic acid is an important C4 platform chemical in the synthesis of many commodity and special chemicals. In the present work, different compounds were evaluated for succinic acid production by Actinobacillus succinogenes GXAS137. Important parameters were screened by the single factor experiment and Plackeet-Burman design. Subsequently, the highest production of succinic acid was approached by the path of steepest ascent. Then, the optimum values of the parameters were obtained by Box-Behnken design. The results show that the important parameters were glucose, yeast extract and MgCO3 concentrations. The optimum condition was as follows (g/L): glucose 70.00, yeast extract 9.20 and MgCO3 58.10. Succinic acid yield reached 47.64 g/L at the optimal condition. Succinic acid increased by 30% than that before the optimization (36.89 g/L). Response surface methodology was proven to be a powerful tool to optimize succinic acid production.