响应面法优化生防菌吡咯伯克霍尔德氏菌JK-SH007的发酵工艺

Optimization of biocontrol agent Burkholderia pyrrocinia strain JK-SH007 fermentation by response surface methodology

摘要:

以吡咯伯克霍尔德氏菌Burkholderia pyrrocinia JK-SH007为出发菌株，对其发酵工艺进行优化，以期提高发酵效率。通过筛选试验、最陡爬坡试验和响应面分析确定影响JK-SH007菌株生长最重要两因素为玉米浆和葡萄糖，其最佳浓度分别为13.88 g/L和3.37 g/L。优化后的发酵工艺培养该菌浓度可达1.18×109 CFU/mL，比优化前提高1.35倍，抑菌活性提高28.84％。

Abstract:

In order to improve ferment efficiency of biocontrol agent Burkholderia pyrrocinia JK-SH007, the fermentation conditions of this strain were optimized. The optimal fermentation conditions were corn steep liquor (13.88?g/L) and glucose (3.37 g/L) by screening test, steepest ascent experiments and response surface analysis. The results showed that the cell density of JK-SH007 (1.18×109 CFU/mL) increased 1.35 times than before, and there was a 28.84% increase in antifungal activity.