生物电化学系统还原降解氯霉素

Reductive degradation of chloramphenicol in bioelectrochemical system

摘要:

为了探讨低温 (12±2 ℃) 条件下还原降解硝基芳香类抗生素氯霉素，采用序批式生物电化学系统(Bioelectrochemical system，BES) 阴极还原的方式 (外加0.5 V电压)，主要研究氯霉素在BES生物阴极与非生物阴极中的不同降解速率、代谢途径和氯霉素在电化学系统中被还原为胺类产物从而脱除细菌抗性。实验数据表明，BES反应器整体的欧姆内阻随着磷酸盐缓冲液浓度的增加而减小；当葡萄糖和污泥发酵液分别存在时，生物阴极24 h的氯霉素还原效率分别为 (86.3±1.69)％和 (74.1±1.44)％，而相同条件下的非生物阴极24 h氯霉素还原效率仅为 (57.9±1.94)％。研究结果表明，生物电化学系统还原降解氯霉素使其失去生物抗性是可行的，并且在低温地区含氯霉素废水的处理过程中，生物阴极是极具潜力的一项处理工艺。

Abstract:

In this study, we investigated reductive degradation of nitroaromatic antibiotic chloramphenicol to non-effective antibacterial amine product in fed-batch biocatalyzed electrolysis systems (BES) (applied voltage was 0.5 V) under low temperature (12±2 °C). The ohm resistance of the whole BES reactor increased when the phosphate buffer solution concentrations decreased. Efficiencies (ErCAP) of chloramphenicol reduction with biocathode (PBS, 25 mmol/L) in presence of glucose was (86.3±1.69)% within 24 h and sludge fermentation liquor was (74.1±1.44)% within 24 h. While the ErCAP of abiotic cathode under the same condition was only (57.9±1.94)% within 24 h. It suggested that biocathode could be a promising technology for reductive biodegradation of nitroaromatic antibiotics-containing wastewater in areas with relatively low annual mean temperature.