聚乙二醇修饰重组细胞珠蛋白对小鼠急性肝损伤的保护作用

Protective effects of PEG modified recombinant cytoglobin on acute liver injury in mice

摘要:

旨在探究聚乙二醇修饰重组细胞珠蛋白 (PEG modified recombinant cytoglobin，PEG-rCygb) 对小鼠急性肝损伤的保护作用。采用CCl4诱导KM小鼠急性肝损伤模型，尾静脉注射PEG-rCygb，收集血清及肝脏组织检测各项生化指标及组织病理学变化。结果表明，PEG-rCygb治疗组小鼠肝脏系数减小, 血清中AST﹑ALT水平降低, 肝组织匀浆中MDA含量减少, GSH含量增加，T-SOD、CAT活性升高。肝组织切片HE染色显示PEG-rCygb可以缓解肝细胞脂肪变性, 减少炎症因子, 减轻肝细胞损伤。体外细胞学实验表明rCygb经PEG修饰后对H2O2造成的肝星状细胞 (HSC) 氧化损伤发挥的保护作用增强。研究结果显示PEG-rCygb提高了机体对自由基的清除能力, 对CCl4引起的小鼠急性肝损伤具有保护作用。

Abstract:

To investigate the protective effect of polyethylene glycol (PEG) modified recombinant cytoglobin (PEG-rCygb) on acute liver damage in mice. The acute liver injury model of KM mice was induced by CCl4 and then treated with PEG-rCygb, The liver and blood samples were collected for biochemical and histopathological analysis. The results showed that PEG-rCygb reduced the liver mass index and decreased significantly the levels of alanine amiotransferase (AST) and aspartate transaminase (ALT) in mouse serum. In liver tissues, the content of malondialdehyde (MDA) was decreased, whereas the content of glutathione (GSH) was increased in PEG-rCygb treated group. PEG-rCygb also elevated the activities of total super oxidedismutase (T-SOD) and catalase (CAT) in liver tissues. HE staining of liver tissue slices revealed that PEG-rCygb relieved fatty degeneration of liver, decreased inflammatory factors and reduced liver cell injury. Further in vitro experiments indicated that the protective effects of PEG-rCygb on hepatic stellate cell (HSC) against H2O2 were enhanced compared with that of rCygb. All results indicated that the PEG-rCygb promoted oxygen free radical scavenging ability and prevented acute liver injury in KM mice induced by CCl4.