热休克蛋白27增强A型流感病毒NS1对β干扰素的抑制作用

Heat shock protein 27 enhances the inhibitory effect of influenza A virus NS1 on the expression of interferon-β

摘要:

热休克蛋白27 (Heat shock protein 27，HSP27) 是一种具有多重功能的小热休克蛋白，它在一些病毒的生命周期中也发挥着重要作用。为研究HSP27对流感病毒感染的调节作用，首先在原核及真核细胞中克隆并表达了人源的HSP27蛋白，并验证了HSP27和A型流感病毒NS1蛋白能够相互结合。通过荧光素酶检测试验发现，HSP27可以抑制病毒感染细胞中β干扰素 (IFN-β) 的表达，但不依赖于其自身的磷酸化状态，而且HSP27与NS1共同对于IFN-β的表达具有叠加抑制效果。进一步的结果表明HSP27可能通过RIG-I样RNA解旋酶 (RLH) 途径中MDA5因子抑制IFN-β的表达。研究表明，HSP27在被感染细胞的天然免疫中发挥一定作用，有助于进一步阐明宿主因子对于流感病毒感染的调节机理。

Abstract:

Heat shock protein 27 (HSP27) is a member of the small heat shock proteins (sHSP) and has multiple functions, it also plays an important role in the life cycle of some viruses. To investigate the regulatory effect of HSP27 during influenza virus infection, we cloned and expressed human HSP27 in both prokaryotic and eukaryotic cells, and demonstrated that HSP27 interacted with influenza A virus NS1 protein both in vivo and in vitro. Luciferase assay showed that HSP27 inhibited the expression of interferon-β (IFN-β) in infected cells, and independent of its phosphorylation. Moreover, HSP27 enhanced the inhibitory effect of NS1 on the expression of IFN-β. Further analysis indicated that HSP27 exerted the inhibitory effect probably through influencing MDA5 of the RIG-I like helicase (RLH) pathway. The results suggested that HSP27 play a role in the innate immunity of infected cells, contributed to our understanding of the regulatory effect of host factors during influenza virus infection.