基于AFLP标记的野生梅种质的鉴定

Identification of the wild germplasm of Prunus mume based on AFLP markers

摘要:

梅Prunus mume是我国原产的名花佳果。为有效保护和利用野生种质资源，文中采用了AFLP标记技术，结合形态表型特征分析，对65份野梅种质进行试验分析。首先从64对引物中筛选出MseⅠ-EcoRⅠ8对引物组合，扩增出1 002条多态性条带。按照Nei’72距离系数进行聚类，在Nei’72=0.26处，可区分西山野梅原变种、西山野梅原种、西山毛梅、厚叶梅、南大坪桃梅、嵩明小梅、曲梗常绿梅、蜡叶梅以及匍匐梅，这与形态学上变种或变型的划分一致。群体内变种单株样本遗传多样性丰富。基于梅花种质资源的遗传变异，建

Abstract:

Mei (Prunus mume Sieb.et Zucc.) is traditionally not only a famous special ornamental plant but also a fruit tree origined in China. In order to conserve and ultilize scientifically the germplasm resources of wild mei, we identified and analysed the germplasm of mei flower in 65 samples collected from the habitat, using AFLP makers in combination with morphological anaylsis. This study amplified clearly 1 728 polymorphic bands, using the 8- pair-primer of MseⅠ-EcoRⅠscreened totally from 64 -primer combination. According to the Nei' 72 distance coefficient clustering, all of the formas and varieties used in this study, including Prunus mume var. mume, P. mume, P. mume var. goethartiana, P. mume var. pallescens, P. mume var. microcarpa, P. mume var. cenrnus-sempervirens (newly recoeding variety), P. mume var. cernua, P. mume var. pallidus, P. mume var. taomei, were identified at the point of Nei' 72=0.26. Due to the genetic difference obviously among the formas and varieties, we suggested that wild germplasmtypes of all formas and varieties in P. mume should be conserved in their habitats in the furture.