重组海洋细菌漆酶Lac15脱色人工合成纺织染料的潜力

Dye decolorization by bacterial laccase Lac15

摘要:

以重组海洋细菌漆酶Lac15 (rLac15) 为生物催化剂，对蒽醌类和偶氮类染料进行脱色，考察了rLac15对人工合成纺织染料的脱色潜能。通过研究催化的介体、酶量、pH、染料浓度以及温度对脱色效率的影响，进一步优化了rLac15对部分蒽醌类和偶氮类人工合成纺织染料的脱色条件。以丁香酸甲酯为介体，在pH 8.5、45 ℃条件下反应1 h，20 U/L rLac15对100 μmol/L偶氮染料类Acid Red 6B (AR-6B)，Reactive Blue 194 (M-2GE)，Reactive B

Abstract:

We screened for laccase from a marine metagenomic library and obtained a bacterial laccase Lac15 and studied its decolorization ability. Using synthetic azo dyes and anthraquinonic dyes as substrates, we investigated the dye decolorization ability of recombinant Lac15 (rLac15). The purified rLac15 had better decolorization ability towards the azo dyes than the anthraquinonic dyes. When incubated at 45 oC and pH 8.5 for 1 h with methylsyringate as the mediator, 20 U/L of rLac15 could decolorize 95% of 100 μmol/L Acid Red 6B (AR-6B), 93% of Reactive Blue 194 (M-2GE), 76% of Reactive Brilliant Orange (K-7R) and 66% of Reactive Blue 171 (KE-R). The decolorization ability of rLac15 decreased with the dye concentration increasing. However, more than 80% of M-2GE and AR-6B were degraded even when the dye concentration was up to 200 μmol/L. At room temperature, rLac51 exhibited significant decolorization ability, with 96% of AR-6B, 86% of M-2GE, 66% of K-7R and 66% of KE-R degraded within 24 h at 25 oC. rLac15 has the potential of industrial applications.