过表达miR-103促进猪前体脂肪细胞分化

Over-expressed MiR-103 promotes porcine adipocyte differentiation

摘要:

为阐明miR-103在猪前体脂肪细胞分化过程中的调控作用，采用Real-time PCR检测猪前体脂肪细胞成脂分化过程中的miR-103表达谱，明确了其在分化过程中的表达趋势；使用miR-103的腺病毒超表达载体感染猪原代脂肪细胞，随后采用Real-time PCR和Western blotting分别检测成脂标记基因PPARγ、aP2的mRNA和蛋白表达量变化；油红O染色观察腺病毒miR-103侵染的前体脂肪细胞诱导分化第8天的成脂情况。结果显示，miR-103的表达量随着脂肪细胞分化而增加，在miR-

Abstract:

To clarify the function of miR-103 in the differentiation of porcine preadipocyte , we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker—PPARγ and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARγ, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.