高等植物葡萄糖-6-磷酸脱氢酶的研究进展

Research progress in glucose-6-phosphate dehydrogenase in higher plants

摘要:

葡萄糖-6-磷酸脱氢酶是植物戊糖磷酸途径中的一个关键性调控酶。其主要生理功能是产生供生物合成需要的NADPH及一些中间产物；此外还参与各种生物和非生物胁迫的应答反应。文中主要从葡萄糖-6-磷酸脱氢酶同工酶与调节机制等方面探讨了其生物学功能，再从胁迫耐受、基因克隆、酶的缺失和替代等方面的研究进行综述, 并对已发表的高等植物中的G6PDH氨基酸序列进行聚类分析，为今后该酶的研究提供参考。

Abstract:

Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the first and rate-limiting step of the oxidative pentose phosphate pathway, existing in both cytosolic and plastidic compartments of higher plants. Its main function is to provide reducing power (NADPH) and pentose phosphates for reductive biosynthesis and maintenance of the redox state of the cell. In addition, the expression of this enzyme is related to different biotic and abiotic stresses. In this review, we analyzed the isoenzyme, regulation and biological function of G6PDH. Meanwhile, we summarized the progress work of G6PDH involved in stress resistance, gene cloning, enzyme-deficiency and cluster analysis. Problems should be solved were also discussed.