激素对转基因雪莲毛状根植株再生及类黄酮产生的影响

Effects of phytohormones on plant regeneration and production of flavonoids in transgenic Saussurea involucrata hairy roots

摘要:

为了研究外源激素GA₃ 和IAA 对3 个转基因新疆雪莲类黄酮高产毛状根系C17、C27、C46 的植株再生及其总黄酮含量的影响，在培养基中添加不同浓度的GA₃ 和IAA，结果发现，GA₃ 浓度高于1.0 mg/L 时，可诱导毛状根系产生不定芽，其中以GA₃ 浓度为2.0 mg/L 时，转基因毛状根系C17 的不定芽再生率最高，可达82％。高压液相色谱以及紫外分光光度法测定结果表明，与未用激素处理的毛状根和它的再生植株相比，外源激素GA₃ 和IAA 能显著提高毛状根培养物中芹菜素和总黄酮的含量。毛状根系的组织干重与类黄酮的含量没有相关性，但毛状根系的再生率与类黄酮的含量几乎呈反相关性。

Abstract:

We investigated the plant regeneration and production of flavonoids in three high-yield flavonods transgenic Saussurea involucrata hairy roots C17, C27 and C46 by quantification of two phytohormones GA₃ and IAA. The results showed that GA₃ concentration at more than 1.0 mg/L could induce adventitious shoots in the hairy root lines. The highest shoot regeneration rate, about 82%, was obtained when the hairy roots C17 were cultured with 2.0 mg/L GA₃. The results on HPLC and UV spectrophotometry showed that exogenous application of both GA₃ and IAA increased the content of flavonoids in the hairy roots. The contents of flavonoids and apigenin in the hormone-treated hariy roots and regenerates were higher comparing with those in the untreated hairy roots and the regenerates. However, the content of flavonoids was not related to tissue weight, and was negatively related to the regeneration efficiency.