拟南芥FRUITFULL (FUL) 基因的表达调控模式

Regulation pattern of the FRUITFULL (FUL) gene of Arabidopsis thaliana

摘要:

FRUITFULL (FUL) 基因是一类MADS box基因，在控制开花时间、花分生组织分化、茎生叶形态以及心皮和果实的发育中发挥重要作用。为了阐明FUL的表达调控模式，克隆了拟南芥Arabidopsis thaliana FUL启动子区(?2 148 bp~+96 bp) 及其第一内含子，并构建一系列启动子分段缺失表达载体及含FUL第一内含子的融合载体。并进一步构建了各顺式作用元件融合拟南芥TUBULIN和ACTIN启动子的表达载体。转基因拟南芥分析结果表明，FUL启动子的上游存在2个抑制其表达的顺式作用元件，其中一个很可能与转录因子AP1的结合有关；2个存在于上游调控区的CArG-box对FUL基因表达起到重要的调控作用；FUL基因第一内含子参与拟南芥心皮和雄蕊的发育调控，而且有增强基因表达的作用。

Abstract:

FRUITFULL (FUL) is an MADS box gene that functions early in controlling flowering time, meristem identity and cauline leaf morphology and later in carpel and fruit development in Arabidopsis thaliana. In order to clarify the regulation of FUL expression the upstream regulatory region, ?2 148 bp?+96 bp and the first intron of the FUL gene were cloned, and vectors with a series of deletion of FUL promoter, and the ones fused with the first intron were constructed. Vectors harboring the fusion of cis-acting elements with the constitutive promoters of TUBULIN and ACTIN were also constructed. β-Glucuronidase activity assays of the transgenic Arabidopsis plants showed that two cis-elements were involved in the repression of FUL expression, with one of the two being probably the binding site of the transcriptional factor AP1. And the two CArG boxes played a important role in FUL initiation particularly. Furthermore, the first intron of FUL was shown to participate in the development of carpel and stamen as an enhancer.