三个小麦春化基因的时空表达特性分析

Spatiotemporal expression patterns of three vernalization genes in wheat

摘要:

为明确小麦春化基因的时空表达特性，以中国春和洛旱2号小麦品种为试验材料，利用半定量RT-PCR技术，分析了3个春化基因VERNALIZATION1 (VRN1)、VRN2和VRN3的时空表达特性。结果表明，VRN1在中国春的三叶期叶片和根、灌浆期的茎秆和旗叶、花药、胚珠和发育的种子中均有不同程度的表达。在开花前，表达水平呈上升趋势，而花后呈降低的趋势，在干种子和萌发种子的胚芽中没有检测到表达；在洛旱2号中，除了在三叶期的叶片和根中没有检测到表达外，VRN1的表达特性与中国春有相同的趋势。VRN2只在三叶期的叶片和萌发种子的胚芽中表达，在其他检测的组织中没有表达；VRN3的表达与VRN1的时空表达特性相似，但在根中未检测到表达。这一结果为进一步分析普通小麦品种春化发育的分子调控机理提供了重要信息。

Abstract:

To identify spatiotemporal expression patterns of vernalization genes in common wheat, we analyzed expression characteristics of several vernalization genes (VRN1, VRN2 and VRN3) in the wheat cultivars ‘Chinese spring’ and ‘Luohan 2’ by RT-PCR. The VRN1 gene was expressed at different levels in the leaves and roots at the 3-leaf stage, stems, flag leaves at the grain-filling stage, anthers, ovules, and developing seeds in ‘Chinese spring’. Expression of VRN1 increased before flowering date, then decreased after flowering time. Expression of VRN1 was not detected in dry seeds or seeds germination. Expression patterns of VRN1 in ‘Luohan 2’ were similar to those in ‘Chinese spring’, except that it was not expressed in roots or in the leaves at the 3-leaf stage in ‘Luohan 2’. Expression of VRN2 was only detected in the leaves at the 3-leaf stage and in the embryo buds during seeds germination. The spatiotemporal expression of VRN3 was similar to that of VRN1, except that VRN3 was not expressed in roots. These results improved our understanding of the molecular regulation of vernalization genes in common wheat.