高温油藏采出液中嗜热产甲烷古菌的分离鉴定

Isolation and identification of a methanogen from the high temperature oil reservoir water

摘要:

为了进一步从高温油藏中发掘新的微生物种质资源，采用 Hungate 厌氧操作技术从大港油田油井采出水中分离出一株嗜热自养产甲烷杆菌DL-7。生理生化结果显示菌株DL-7只能够利用H2/CO2生长，不利用甲酸、甲醇、三甲胺、乙酸和二级醇类；最适生长温度60℃；最适盐浓度0.8 g/L；最适pH为7.0~7.5；只有在添加酵母粉的培养基中才可以较好生长。16S rRNA序列比对结果显示菌株DL-7与标准株M. marburgensis DSM 2133T(X15364) 的16S rRNA基因序列相似性为99.7％。

Abstract:

To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60°C, pH 7.0?7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T (X15364).