拮抗链霉菌S24抗菌物质的提取及其部分理化性质

Extraction and characterization of antifungal substances produced by antagonistic Streptomyces S24

摘要:

拮抗链霉菌S24对黄曲霉、赭曲霉、黑曲霉等粮食和饲料中常见的曲霉菌具有广谱抗性，本试验通过牛津杯法测定抗菌物质的效价，研究了大孔吸附树脂对链霉菌S24产生的抗菌物质的吸附、解吸性能，筛选了解吸剂，并研究了抗菌物质的部分理化性质。结果表明，大孔吸附树脂AB-8对抗菌物质的吸附及解吸性能最好，其饱和吸附量为7.0822×104 μg/g，最佳解吸剂为85％丙酮，以85％丙酮进行动态解吸，解吸率达93.82％。该抗菌物质对热稳定，对紫外线敏感，对有机溶剂不敏感，对酸敏感，对碱稳定，紫外全波长扫描发现该抗菌物质为多烯大环内酯类抗生素。

Abstract:

Streptomyces S24 has broad spectrum resistance to the Aspergillus in food and feed, such as Aspergillus flavus, Aspergillus niger, Asperegillus alutacells and so on. We studied the adsorption and desorption properties of antifungal substance from Streptomyces S24 on macroporous resins, screened the best elution solution and also investigated some physical and chemical characters of antifungal substance by determining the antifugal activity using oxford plate assay system. According to the analysis results, AB-8 resin offered the best adsorption and desorption capacity for antifungal substance and its saturated absorption capacity was 7.0822×104 μg/g, the optimal elution solution was 85% acetone and the dynamic desorption rate could reach 93. 82%. The antifungal substance was stable to heat and alkali, not sensitive to organic solvents, and sensitive to ultraviolet rays and acid. Based on its ultraviolet spectrometry, the antifungal substance was identified as heptaene macrolide antibiotic.