里氏木霉和鸡腿菇利用秸秆共发酵产木质降解酶

Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei

摘要:

为了更好地利用农业废弃物, 提高其综合利用率, 减少传统化学方法及秸秆焚烧过程造成的环境污染, 实验对鸡腿菇、黑曲霉和里氏木霉3株产木质纤维素降解酶系的菌株进行混合平板产酶筛选, 结果显示鸡腿菇和里氏木霉平板培养相容性良好, 且产酶量高。在相容性实验的基础上, 对鸡腿菇和里氏木霉的最优产酶条件进行了研究。在最优条件下: 鸡腿菇和里氏木霉接种比例按5: 2, 接种时间间隔为12 h, 26oC、150 r/min下, 发酵3 d产漆酶活力达3267.2?U/mL, 比单独发酵提高106%。

Abstract:

In order to enhance the utilization efficiency, reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration; we studied the ligninase production by Coprinus comatus, Aspergillus niger and Trichoderma reesei through the plate screening. The results showed that C. comatus mixed culture with T. reesei have a good compatibility and higher yields of Laccase. On the basis of this pre-experiment, we studied the optimal conditions of mixed culture for enzyme production. Under the optimal conditions: the inoculation proportion of C. comatus and T. reesei (5:2), the interval of time (12?h), the temperature 26oC, the shake rotation speed 150 r/min, fermented for 3 days, the Laccase activity reached 3267.1 U/mL, increased by 106% contrasted with single culture of C. commatus.