应用荧光分析法检测酶的研究进展

Recent advances in enzyme assays using fluoremetry

摘要:

酶是细胞新陈代谢的基础, 酶的检测在生物技术、疾病诊断及药物开发等领域都具有十分重要的意义。在检测酶的诸多方法中, 荧光法因其灵敏度高、检测限低等优势发展迅速。以下简述了近年来荧光法在酶检测领域的研究, 根据检测方法的不同分为直接荧光检测法和间接荧光检测法, 其中直接检测法又根据不同的底物标记及检测机理进行分类。以下介绍了各种方法的应用, 并展望了此类方法的前景和发展趋势, 为酶工程及生命科学其他领域的相关研究提供信息。

Abstract:

Enzymes play such a pivotal role in cellular metabolism that enzyme assays are important for bio-engineering, disease diagnoses and drug discovery. Among the reported methods, fluoremetry has attracted more and more attention due to its high sensitivity and possibility of continuous dynamic monitoring. The recent progresses and applications in enzyme assays using fluorescent probes were reviewed. Different methods were classified into direct fluorescence detection and indirect fluorescence detection according to their labeled substrates and detection mechanisms. Our writing purpose is to provide the readers with a flavor of the kinds of tools and strategies available in enzyme assays with fluorescent probes. Also, the research situation and prospects were discussed.