类人胶原蛋白-丝素蛋白血管支架的制备及性能表征

Preparation and properties of novel human-like collagen-silk fibroin scaffold for blood vessel

摘要:

为了提高血管支架的力学性能, 将生物相容性良好的新型生物材料类人胶原蛋白(基因工程技术、高密度发酵生产)与丝素蛋白以质量比9:1、7:3、5:5复合, 采用真空冷冻方法制备管状血管支架。研究了不同配比血管支架材料的表面结构、表面元素组成、力学性能、降解和生物相容性。结果表明：当类人胶原蛋白与丝素蛋白的质量比为7:3混合时, 类人胶原蛋白-丝素蛋白管状支架具有均匀的多孔结构, 孔径为(60±5) μm, 孔隙率达到85%以上; 获得了较理想的力学性能：应变为50%±5%, 应力为(332±16) kPa; 具有相对慢的降解速率; 提高了细胞的黏附与增殖, 具有良好的生物相容性。

Abstract:

In order to improve tensile property of vascular scaffold, we blended silk fibroin with novel human-like collagen with the mass ratio of 9:1, 7:3 and 5:5 (W/W), and then fabricated blood vessel tubular graft by freeze-drying process. We studied microstructure, mechanical properties, elements composites, degradability and biocompatibility of vascular scaffolds. These results showed that tubular scaffold with mass ratio 7:3 exhibited interconnected porous structure with pore size at (60 ± 5) μm and porosity of 85%; achieved the desirable mechanical property (strain of 50% ± 5% and stress of 332 ± 16 kPa); had relatively slow degradation rate; could enhance cell adhesion and proliferation and had superior biocompatibility.