表达抗-CD25单克隆抗体的GS-NS0骨髓瘤细胞无血清培养及代谢特性

Metabolic characteristics of GS-NS0 Myeloma cells producing anti-CD25 monoclonal antibody in serum-free culture

摘要:

抗-CD25单克隆抗体作为免疫抑制剂拥有广阔的市场前景和巨大的经济价值。本实验以表达抗-CD25单克隆抗体的GS-NS0细胞为研究对象, 开发了支持其大规模培养和抗体表达的无血清低蛋白培养基, 批培养最大活细胞密度和最大抗体浓度分别达3×106 cells/mL和300 mg/L以上, 比商业无血清培养基(Excell 620 + 0.2% primatone)分别提高了100%和46%。通过批培养实验, 研究了细胞的生长、葡萄糖和氨基酸代谢、以及产物表达特点, 并揭示了批培养过程中初始葡萄糖浓度对GS-NS0细胞生长与代谢的影响规律。为优化GS-NS0细胞培养过程和抗CD25单抗成功迈向产业化提供了重要的科学依据。

Abstract:

As an immunodepressant, anti-CD25 monoclonal antibody has a huge market with wide prospect and economic value. We developed a low protein serum-free medium for large-scale GS-NS0 myeloma cell culture and anti-CD25 monoclonal antibody production. Further study focused on the characteristics of GS-NS0 cell growth, glucose and amino acid metabolism, and antibody production. In the serum-free medium, the maximal viable cell density and antibody concentration reached above 3×106 cells/mL and 300 mg/L in batch culture. Compared with the commercial serum-free medium (Excell 620 + 0.2% Primatone), the maximal viable cell density doubled and the maximal antibody concentration increased 46%. Results also showed the specific growth rate decreased when the glucose concentration was lower than 6 mmol/L. And the production of lactate increased when glucose concentration was excessively high (>30 mmol/L). These results were important to provide technique and theory basis for developing optimized GS-NS0 cell culture and anti-CD25 monoclonal antibody production processes.