水杨酸对霍山石斛类原球茎细胞生长及多糖合成的影响

Effect of salicylic acid on cell growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense

摘要:

本研究考察了水杨酸对霍山石斛类原球茎悬浮培养细胞生长、多糖合成、碳代谢的影响, 并研究了细胞生长、多糖合成以及蔗糖消耗的动力学。结果表明, 水杨酸对霍山石斛类原球茎细胞生长有轻微的抑制作用, 但能够显著改善类原球茎对碳源的利用, 提高胞内可溶性糖的含量, 从而促进多糖的合成, 其中以添加100 mmol/L浓度的水杨酸效果最好, 培养18 d时, 多糖产量达到3.129 g/L, 为对照的1.63倍。建立的培养动力学模型能较好地反映水杨酸调控霍山石斛类原球茎悬浮培养过程中细胞生长、多糖合成和蔗糖消耗特性。

Abstract:

Polysaccharides from Dendrobium huoshanense possess immunostimulating activity, antioxidant activity and anticataract activity. In order to produce the active polysaccharides from Dendrobium huoshanense through cell culture, we investigated the effects of salicylic acid on cell growth, accumulation of polysaccharides and utilization of carbon source in suspension cultures of protocorm-like bodies from Dendrobium huoshanense. Although salicylic acid slightly inhibited the cell growth, it was beneficial to the utilization of carbon source, thus leading to significant increase in the contents of polysaccharides. The highest polysaccharide production occurred on the medium supplied with 100 μmol/L salicylic acid. After 18 days of culture the production of polysaccharides reached 3.129 g/L, which was 1.63 times that of the control. Further, we established the kinetic models describing cell growth, polysaccharide production and carbon source utilization based on Logistic equation, Luedeking-Piret equation and Luedeking-Piret-Like equation. The calculated values from the kinetic models showed a good fit to the experimental values, suggesting that salicylic acid could be an effective compound to enhance the production of active polysaccharides from protcorm-like bodies from Dendrobium huoshanense.