小麦肽对受环磷酰胺免疫抑制小鼠的免疫调节及抗氧化功能

Immune modulation and antioxidant effects of wheat peptides on immunosuppressed mice

摘要:

本研究旨在分析小麦蛋白活性肽对免疫抑制小鼠免疫功能和抗氧化功能的调节作用。小鼠灌胃小麦肽10 d, 第8天用环磷酰胺诱导免疫抑制, 测定血清溶血素、抗体生成细胞含量、脾细胞增殖、体外腹腔巨噬细胞吞噬能力、肝脏抗氧化酶活性和MDA以及血清清除DPPH和·OH的能力。实验结果表明, 环磷酰胺处理显著的降低了小鼠血清中抗SRBC抗体(溶血素HC50)水平和腹腔巨噬细胞的吞噬能力; 同时伴随着肝脏超氧化物歧化酶活性(SOD)、过氧化氢酶活力(CAT)、总抗氧化能力(T-AOC)的降低和丙二醛(MDA)含量的提高。给小鼠灌胃小麦肽可以恢复HC50和脾细胞增殖, 显著提高抗体生成细胞含量和腹腔巨噬细胞吞噬能力; 此外, 小麦肽增强了小鼠血清清除DPPH和清除羟自由基(·OH)的能力。以上结果表明, 小麦肽可以调节应激状态引起的机体抗氧化体系紊乱及免疫功能的降低。这可能与小麦肽缓冲自由基生成、激活腹腔巨噬细胞和脾淋巴细胞活性有关。

Abstract:

We studied immune modulation and antioxidant effects of wheat peptides on immunosuppressed mice. Mice were administrated with wheat peptides orally for 10 days and treated with cyclophosphamide at the 8th day. The indexes including serum hemolysin, plaque forming cells, spleen cells proliferation, liver antioxidant enzymes activties, MDA, scavenging serum 2,2-Diphenyl-1-picrylhydrazyl and ·OH and macrophage phagocytic ability in vitro were measured to assess the immune functions and antioxidation abilities. In vivo study shows that cyclophosphamide significantly decreases serum hemolysin (HC50) and phagocytic function of macrophages. Simultaneously, liver superoxide dismutase, catalase activity and total oxidation capacity were decreased and malondialdehyde was increased. Wheat peptides could recover HC50 and spleen cell proliferation when orally administrated. Furthermore, they could also enhance serum 2,2-Diphenyl-1-picrylhydrazyl and ·OH scavenging. In conclusion, wheat peptides can help body resist the stress related disorders in immune and antioxidant system.