反义技术应用在细菌代谢调控中的研究进展

Progress of Antisense Technology Applied in Metabolic Regulation of Bacteria

摘要:

随着基因工程技术的蓬勃发展和代谢调控研究的深入, 反义技术作为一种温和调控的基因工程技术, 开始向世人展示其无穷的魅力。与基因敲除等功能缺失性研究方法相比, 反义技术具有投入少、周期短、操作简单等优点, 受到广泛的关注, 成为细菌代谢调控的有力工具。以下对反义RNA、反义寡核苷酸、核酶这几种反义技术在细菌代谢工程操作中的研究进展及存在的问题进行了概述。

Abstract:

With the rapid development of genetic engineering and metabolic regulation, antisense technology displays its fascination to the world as a mild regulation genetic tool. Compared with other loss-of-function research methods (e.g. gene knockout), antisense technologies have advantages such as low cost, short period, and easy operation. It has been increasingly used in bacterial metabolic regulation as a powerful genetic tool. This review briefly summarized the latest progress and problems in antisense technologies that are recently used in metabolic engineering of bacteria, and compares the advantages and disadvantages of these technologies.