拟南芥鼠李糖合成酶基因AtRHM1启动子区葡萄糖应答调控元件的鉴定

Identification of Glucose-responsive Elements in the Promoter of UDP-L-rhamnose Biosynthesis Gene RHM1 in Arabidopsis thaliana

摘要:

植物中, UDP-L-鼠李糖是细胞壁骨架的主要成分, 由鼠李糖合成酶催化底物UDP-a-D-葡萄糖合成。本实验从拟南芥基因组中分离了鼠李糖合成酶基因AtRHM1 1058 bp的启动子序列并对启动子5￠端进行了不同长度的缺失。将全长启动子及不同缺失启动子与GUS报告基因进行融合后转化野生型拟南芥, 获得了一系列转基因植株。启动子缺失分析结果表明, AtRHM1基因在转录水平上受葡萄糖的诱导, 参与葡萄糖应答反应的顺式调控元件位于启动子的-931 bp~ -752 bp区域。

Abstract:

In plants, UDP-L-rhamnose is one of the major components of cell wall skeleton. Rhamnose synthase plays a key role in rhamnose synthesis which converts UDP-D-glucose into UDP-L-rhamnose in plants. In this study, we isolated the 1058 bp promoter region of the rhamnose synthase gene AtRHM1 from Arabidopsis genome by PCR, and created a series of deletions of AtRHM1 promoter ranging from -931 bp to +127 bp. The full length of the promoter and its deletion derivatives fused with GUS reporter gene were introduced into wild-type Arabidopsis by Agrobacterium-mediated transformation respectively. The GUS staining and GUS enzymatic activity assay showed that the expression of AtRHM1 is induced at transcriptional level by glucose and the regulatory elements involved in the glucose response are located in the region of -931 bp ~ -752 bp which contains three G-box motifs.