转trxS基因大麦发芽种子水解酶活性的变化

Change of Hydrolase Activity in Germinating Seeds of trxS Transgenic Barley

摘要:

利用转基因技术是改良大麦品种品质的有效途径。研究了转trxS基因对大麦种子发芽过程中水解酶活性的影响, 结果表明转基因种子中a-淀粉酶、自由态b-淀粉酶和极限糊精酶的活性比未转基因种子高; 转基因种子醇溶蛋白和谷蛋白中巯基的含量提高, 说明该基因能够表达, 为大麦育种和品质改良提供新的途径。

Abstract:

Genetic modification of barley variety can be an efficient way to improve beer quality. The objective of this study was to understand the effect of trxS gene on hydrolases activities in transgenic and non-transgenic barley seeds. The results showed that a-amylase, free b-amylase and limit dextrinase activity were increased in transgenic seeds in comparison with non-transgenic seeds. Sulfhydryl content of protein in transgenic seeds was also higher than that in non-transgenic seeds, suggesting that trxS gene could express in barley seeds, which opens a new way for breeding new barley varieties to improve beer quality.