谭清苏铁性别连锁的RAPD和SCAR分子标记

RAPD and SCAR Molecular Markers Linked to the Sexuality of Cycads (Cycas tanqingii D. Y. Wang)

摘要:

利用RAPD(Random amplified polymorphic DNA)分子标记技术，寻找谭清苏铁（Cycas tanqingii）中与性别相关的分子标记，筛选了160个10bp的随机引物，产生了2500多个RAPD条带。只有引物S0465 (CCCCGGTAAC)产生了一条大约500bp的雌性特异RAPD标记，该分子标记出现在所有的供试雌性植株中，而所有的供试雄性植株都不具有该标记。对该特异片段进行了克隆和序列测定，并根据序列分析结果将RAPD标记转化为重复性和特异性更好的特异特征序列扩增区域（SCAR）分子标记，并命名为STQC-S465-483。分子标记的建立可用于谭清苏铁幼苗性别的早期鉴定，为谭清苏铁就地保护和迁地保护提供技术支持。

Abstract:

The random amplified polymorphic DNA (RAPD) technique was used to amplify DNA fragment, aiming at finding markers linked to the sex trait in Cycas tanqingii D. Y. Wang. A total number of 160 random primers were screened in the RAPD-PCR and more than 2500 RAPD fragments were generated from the male or the female plants. One fragment of about 500bp was amplified steadily and repeatedly by the S₀₄₆₅ (CCCCGGTAAC) primer only from female plants but not male plants. The RAPD marker was then converted into female-linked dominant SCAR (Sequence Characterized Amplified Regions) marker named STQC-S465-483. The development of this sex-linked SCAR marker provides a possibility of identifying the sex of Cycas tanqingii before sexual maturation, which is very important to in situ or ex situ conservation.