Vitex glabrata R.Br.悬浮培养细胞的生长条件优化及20-羟基蜕皮激素的生产

Optimization of Cell Growth and 20-Hydroxyecdysone Production in Cell Suspension Culture of Vitex glabrata R.Br.

摘要:

研究了培养基、植物生长调节素以及接种量对Vitex glabrata R.Br.悬浮培养细胞的生长情况以及对20-羟基蜕皮激素形成的作用。当细胞在添加有2.0mg/L BAP (6-苯甲酸嘌呤)和1.0mg/L 2,4-D的Gamborg's B5培养基中培养时细胞生长和20-羟基蜕皮激素的形成达到了最高水平。当接种量为20％PCV（积压细胞体积）时观察到了20-羟基蜕皮激素的最高产量，大约是1.1mg/(L·d)。实验数据也表明当接种量增加到20％PCV时，产量提高了7倍。

Abstract:

The effects of the cultivation media, plant growth regulators and inoculum size on the cell growth and 20-hydroxyecdysone production in suspension cultures of Vitex glabrata R.Br. were investigated. The cell growth and 20-hydroxyecdysone formation reach the highest when cells are cultured in the Gamborg's B5 medium supplemented with 2.0mg/L BAP (6-benzylaminopurine) and 1.0mg/L 2,4-D. The maximum 20-hydroxyecdysone productivity, of about 1.1mg/L/day, was observed in the culture with 20% PCV (packed cell volume) of inoculum size. These data also show that the increment of the inoculum size to 20% PCV could increase the productivity in 7-folds.