口蹄疫病毒受体猪源β6亚基基因的克隆和分子特征

Molecular Cloning and Characteristics of cDNA Encoding Pig β6 Subunit for FMDV Receptor

摘要:

从FMDV试验感染康复猪的舌皮和肺组织中克隆到了整联蛋白β6亚基的基因并对其核苷酸序列和推导的氨基酸序列以及蛋白结构进行了分析，该基因已登录到GenBank中，登录号为EF432729。猪整联蛋白β6亚基基因的编码区含有2367个核苷酸，编码788个氨基酸残基，含有9个潜在的糖基化位点，3个氨基葡聚糖结合位点，一个依赖于cGMP的蛋白激酶磷酸化位点，10个蛋白激酶C磷酸化位点，2个表皮生长因子相似结构域和2个半胱氨酸丰富区。其信号肽由26个氨基酸组成，胞外域由681个氨基酸组成，跨膜区由29个氨基酸组成，胞浆域由52个氨基酸组成。从起始密码子开始，共有11个核苷酸发生了变化，其中2 079位和2256位核苷酸的突变是同义突变，其余9位核苷酸的变化为错义突变。猪β6基因与猕猴、小鼠、挪威大鼠、犬、豚鼠、人、牛和羊的β6基因的核苷酸序列一致性分别为79.5%、84.9%、85.4%、85.2%、88.7%、90.1%、91.9%和91.9%，推导的氨基酸序列一致性分别为93.5%、88.2%、88.5%、88.3%、91.0%、92.8%、93.3%和93.4%。为进一步深入研究FMDV嗜性、与宿主细胞的相互作用、病毒的侵入机制等问题奠定了基础。

Abstract:

In order to study the roles of integrin β6 in Foot-and-Mouth Disease Virus infection, pig integrin β6 was firstly molecularly cloned from RNA of the tongue and lung of recovered pig infected experimentally with foot-and-mouth-disease virus (FMDV), and was compared with the β6 gene of other animals available in GenBank at nucleotide and amino acid leves. GeneBank association number of the β6 gene is EF432729. Pig integrin β6 gene (2367bp) encodes a polypeptide of 788 amino acids consisting of 9 potential N-linked glycosylation sites, 3 Glycosaminoglycan attachment sites, a cGMP-dependent protein kinase phosphorylation site, 10 Protein kinase C phosphorylation sites, 2 EGF-like domains and 2 cysteine-rich regions. Pig integrin β6 subunit has a 26-residue putative signal peptide, a 681-residue ectodomain, a 29-residue transmembrane domain, and a 52-residue cytoplasmic domain. 11 mutant nucleotides were found in β6 gene coding region and 9 amino acids were changed. The nucleotide sequence similarity of integrin β6 gene between rheses monkey, mouse, Norway rat, dog, guinea pig, human, bovine, sheep is 79.5%, 84.9%, 85.4%, 85.2%, 88.7%, 90.1%, 91.9% and 91.9%, and the amino acid sequence similarity is 93.5%, 88.2%, 88.5%, 88.3%, 91.0%, 92.8%, 93.3% and 93.4% respectively. This study will lay a foundation for understanding the interactions of FMDV with receptors.