一种快速提取质粒DNA用于鉴别重组克隆的方法

A Rapid Method for Preparation of Plasmid DNA for Screening Recombinant Clones

摘要:

介绍了一种利用过夜培养的菌液瞬时提取质粒DNA，并用于电泳鉴别含有插入子克隆的方法。事先无需准备许多繁琐的相关试剂，提取质粒的全过程只需3 ～ 5min就可完成，非常适合于做重组克隆的快速鉴别。

Abstract:

A simple and rapid method for preparation of plasmid DNA from overnight incubation was introduced. It does not require any additional reagents; the incubation mixture containing recombinant plasmid DNA was just mixed with H₂O and phenol/chloroform/isoamyl alcohol in certain ratio. After vortexing and spinning of the mixture, the supernatant could be directly loaded onto agarose gel and analyzed using electrophoresis. The whole preparation requires only 3～5 minutes. So to quickly screen recombinant clones, this method is better compared with traditional methods.