产氢菌Enterobacter sp.和Clostridium sp.的分离鉴定及产氢特性

Isolation and Characterization of H2-producing Strains Enterobacter sp. and Clostridium sp.

摘要:

在高温水体中分离得到2株具有较高产氢活性的微生物菌株Z-16和C-32。根据两菌株的16S rDNA序列分析，初步鉴定菌株Z-16为Enterobacter sp.，菌株C-32为Clostridium sp.。研究了起始pH值、反应温度、碳源等对菌株放氢活性的影响。菌株Z-16的最适产氢条件为：反应系统起始pH7.0，反应温度35℃，以蔗糖为产氢底物。在最适条件下，菌株Z-16的氢转化率为2.68mol H₂/mol蔗糖。菌株C-32的最适产氢条件为：反应系统起始pH 8.0，反应温度35℃，以麦芽糖为产氢底物。在最适条件下，菌株C-32的氢转化率为2.71mol H₂/mol 麦芽糖。以葡萄糖为碳源时，菌株Z-16和菌株C-32的氢转化率分别为2.35和2.48mol H₂/mol葡萄糖。

Abstract:

Two hydrogen-producing bacterial strains were newly isolated and identified as Enterobacter sp. Z-16 and Clostridium sp. C-32 by 16S rDNA sequence analysis. Various parameters for hydrogen production, including substrates, initial pH and temperature, have been studied. The optimum condition for hydrogen production of strain Z-16 were achieved as: initial pH7.0, temperature 35℃, sucrose as the favorite substrate. In comparison, The optimum condition for hydrogen production of strain C-32 were obtained as: initial pH8.0, temperature 35℃, maltose as the favorite substrate . Under batch fermentative hydrogen production conditions, the maximal hydrogen conversion rate for strain Z-16 and strain C-32 were 2.68 mol H₂/mol sucrose and 2.71mol H₂/mol maltose, respectively. Using glucose as substrate, the hydrogen conversion rate of strain Z-16 and strain C-32 were 2.35 and 2.48 mol H₂/mol glucose, respectively. This research suggest a good application potential of strain Z-16 and C-32 in the future biological hydrogen production.