霍山石斛类原球茎二步法培养细胞生长和多糖合成的动力学研究

Study on Kinetics of Two-stage Cultivation of Protocorm-like Bodies from Dendrobium huoshanense for Cell Growth and Synthesis of Polysaccharides

摘要:

磷是调控类原球茎细胞生长和多糖积累的有效因素。为了获得较高的多糖产量，根据霍山石斛类原球茎生长和多糖积累的动力学特性，提出了二步培养方式，采用了补料策略，研究了其培养过程的动力学特性，并建立了相关模型。结果表明，采用二步法培养，生物量从28.7g DW/L提高到44.2g DW/L, 多糖产量从1.86g/L提高到5.22g/L，多糖含量从6.4%提高到11.9%。建立的模型基本反映了类原球茎生长和多糖积累的动力学机制。

Abstract:

Phosphate is an effective factor in the regulation of cell growth and accumulation of polysaccharides. The method for higher polysaccharide production has been obtained through feeding cultivation. The two-stage cultivation was proposed according to the characteristic of cell growth and the accumulation of polysaccharides in the suspension cultures of protocorm-like bodies from Dendrobium huoshanense. A kinetic model was developed to describe the cultivation process. The results indicate that the production of biomass and polysaccharides increases from 28.7g DW/L and 1.86g/L to 44.2g DW/L and 5.22g/L, respectively, and the content of polysaccharides increases from 6.4% to 11.9%. With the evaluated model parameters, the model appears to provide a description for the cultivation process.