新疆艾比湖嗜盐菌的细菌视紫红质和16S rRNA基因序列的研究

Studies on Bacteriorhodopsin Gene and Sequence of 16S rRNA Encoding Genes of Halophilic Archaea of Xingjiang Aibi Lake

摘要:

为了研究分析嗜盐古生菌物种与细菌视紫红质（BR）蛋白基因资源，从40份土壤、湖水及淤泥样品中分离出148株嗜盐菌，对其中6株菌采用聚合酶链式反应（PCR）方法对其编码螺旋C至螺旋G的蛋白基因片段和16S rRNA基因进行了扩增，并测定了基因的核苷酸序列。与已报道的相应片段进行对比，ABDH10,ABDH1I和ABDH40中的螺旋C至螺旋G的蛋白与其他菌株差异显著。基于16S rRNA序列的同源性比较以及系统发育学研究表明，ABDH10和ABDH40是Natronorubrum属下的新成员和Natrinema属下的新成员，ABDH40的16S rRNA序列已登录到GenBank，其序列号为AY989910。ABDH11中的螺旋C至螺旋G的蛋白与其他菌株差异显著。

Abstract:

One hundred and forty-eight strains of halophilic archaea were isolated from 40 samples of soil，lake water, and silt. To study and analyze the species and bacteriorhodopsin（BR） protein resource，partial DNA fragments encoding BR protein from helix C to helix G and16S rRNA encoding genes from 6 strains of halophilic archaea were amplified by polymerase chain（PCR），and their DNA sequences were determined. The results indicate that the reduced amino acid sequences of BR protein from helix C to helix G of ABDH11 is obviously different from those of other existing proteins. The results of homology analysis on BR gene and16S rRNA and phylogenetic analysis on 16S rRNA show that strains ABDH10 and ABDH40 are novel members of genus Natronorubrum and Natrinema，respectively; the sequence of ABDH40 was obtained from GenBank and the number of sequence is AY989910. The protein from helix C to helix G of ABDH11 is significantly different from that of other strains.