利用重组大肠杆菌进行寡糖合成的研究进展

Progress of Oligosaccharides Biosynthesis in Recombinant Escherichia coli

摘要:

随着更多寡糖生物学活性的阐明，寡糖合成研究已成为糖生物学研究的热点之一，其中，以重组大肠杆菌作为酶盒或生物反应器，利用Leloir途径合成寡糖的方法，是近年来发展起来的一类重要的寡糖生物合成技术，并取得了较多的进展。将从细菌糖基转移酶的表达和鉴定、糖核苷酸的供给和寡糖的合成途径等几个方面，关注利用细菌功能尤其是利用重组大肠杆菌合成寡糖的研究进展，并分析各技术手段的优缺点及其应用前景。

Abstract:

As more bioactivities of oligosaccharides have been elucidated, researches on biosynthesis of oligosaccharides have drawn more concerns in Glycobiology. A lot of enzymatic methods for the synthesis of oligosacchrides have been developed employing recombinant E. coli expressed glycosyltranferase or synthase of nucleotide-sugar. This review focuses on the recent progress in the production of oligosaccharides using bacteria especially by genetically engineered bacteria. The key point concering the oligosaccharides biosynthesis in recombinant E. coli , such as enzyme expression, NDP-sugar provision and biosynthesis pathway, was discussed.