Screening of a selenium-enriching strain of Pleurotus ostreatus by ultraviolet mutagenesis and its application in nutrient enrichment
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference [23]
  • |
  • Related [20]
  • | | |
  • Comments
    Abstract:

    [Background] The cultivation of selenium-enriching edible fungi in their natural habitats often fails to meet the national standards for nutrient enrichment. [Objective] To screen out a selenium-enriching strain of Pleurotus ostreatus by ultraviolet mutagenesis, so as to provide a foundation for the product development with selenium-enriching edible fungi. [Methods] The strain to be mutated was determined by selenium resistance examination and then exposed to ultraviolet irradiation. The mycelial growth rate and the biological efficiency, total and organic selenium content of fruiting bodies were determined to evaluate the performance of the mutant strain. [Results] In the media with 100 mg/kg and 120 mg/kg selenium, respectively, the strain 120-5 showed the mycelial growth rates of 1.27 cm/d and 1.19 cm/d and the total selenium content of 199.77 mg/kg and 224.15 mg/kg in the fruiting bodies of 120-5. The organic selenium content in the fruiting bodies in the two treatments reached 198.83 mg/kg and 223.56 mg/kg, accounting for 99.53% and 99.74% of the total selenium, respectively. These values met the national standards for nutrient enrichment. However, in the media with 100 mg/kg and 120 mg/kg selenium, the original strain qiu-2020 showed the mycelial growth rates of 1.02 cm/d and 0.88 cm/d, respectively. It is noteworthy that in the medium with 120 mg/kg selenium, the original strain achieved the highest selenium content of 166.53 mg/kg, which fell short of the national standards. In the presence of 120 mg/kg selenium, the biological efficiency of the mutant strain and the original strain reached 92% and 77%, respectively. Remarkably, the agronomic traits of both the mutant and original strains remained unaltered. The whole-genome resequencing results unveiled single nucleotide polymorphism (SNP), insertion-deletion (Indel), and structure variation (SV) between the mutant and original strains. [Conclusion] The strain 120-5 with significant genomic mutations showcased a robust capacity of selenium enrichment, serving as a compelling candidate for nutrient enrichment.

    Reference
    [1] COMBS GF Jr. Selenium in global food systems[J]. The British Journal of Nutrition, 2001, 85(5): 517-547.
    [2] MATIJAŠEVIĆ D, PANTIĆ M, STANISAVLJEVIĆ N, JEVTIĆ S, RAJIĆ N, LEVIĆ S, NEDOVIĆ V, NIKŠIĆ M. Coriolus versicolor mushroom grown on selenium-rich zeolite tuff as a potential novel food supplement[J]. Food Technology and Biotechnology, 2021, 60(1): 67-79.
    [3] 胡婷, 惠改芳, 赵桂慎, 郭岩彬. 富硒食用菌研究进展[J]. 食用菌学报, 2019, 26(1): 68-76. HU T, HUI GF, ZHAO GS, GUO YB. Advances in selenium-enriched edible fungi[J]. Acta Edulis Fungi, 2019, 26(1): 68-76(in Chinese).
    [4] BHATIA P, AURELI F, D’AMATO M, PRAKASH R, CAMEOTRA SS, NAGARAJA TP, CUBADDA F. Selenium bioaccessibility and speciation in biofortified Pleurotus mushrooms grown on selenium-rich agricultural residues[J]. Food Chemistry, 2013, 140(1/2): 225-230.
    [5] 郭洁, 周晓华, 王炯, 魏亮亮, 魏红, 张迪, 孙江南, 贾斌, 刘进玺, 王铁良. 硒营养强化对香菇营养品质影响研究[J]. 食品安全质量检测学报, 2022, 13(23): 7782-7787. GUO J, ZHOU XH, WANG J, WEI LL, WEI H, ZHANG D, SUN JN, JIA B, LIU JX, WANG TL. Effects of selenium nutrition enhancement on the nutritional quality of Lentinus edodes[J]. Journal of Food Safety & Quality, 2022, 13(23): 7782-7787(in Chinese).
    [6] 宋盼盼, 赵妍, 李治平, 董沁, 杨焕玲, 陈明杰. 硒对镉胁迫下大球盖菇菌丝生长的影响[J]. 菌物学报, 2021, 40(9): 2402-2411. SONG PP, ZHAO Y, LI ZP, DONG Q, YANG HL, CHEN MJ. Effects of exogenous selenium on the mycelial growth of Stropharia rugosoannulata under cadmium stress[J]. Mycosystema, 2021, 40(9): 2402-2411(in Chinese).
    [7] 国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品中硒的测定: GB 5009.93-2017[S]. 北京: 中国标准出版社, 2017. National Health and Family Planning Commission, State Food and Drug Administration. National standards of food safety: the determination of selenium in foods: GB 5009.93—2017[S]. Beijing: Standards Press of China, 2017(in Chinese).
    [8] 国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品营养强化剂富硒食用菌粉: GB 1903.22—2016[S]. 北京: 中国标准出版社, 2017. National Health and Family Planning Commission, State Food and Drug Administration. Food safety national standard food nutrition enhancers: Se-enriched edible fungi powder: GB 1903.22—2016[S]. Beijing: Standards Press of China, 2017(in Chinese).
    [9] 周晓东. 北虫草菌丝体诱变及优良菌株筛选[D]. 保定: 河北农业大学硕士学位论文, 2013. ZHOU XD. Mutagenizing of Cordyceps militaris by ultraviolet irradiation on mycelial and selection of fine strains[D]. Baoding: Master’s Thesis of Hebei Agricultural University, 2013(in Chinese).
    [10] 黄春燕, 万鲁长, 张海兰, 单洪涛, 郭惠东, 郭洪军, 周学政, 张柏松. 高温富硒平菇栽培技术研究[J]. 山东农业科学, 2013, 45(11): 63-65. HUANG CY, WAN LC, ZHANG HL, SHAN HT, GUO HD, GUO HJ, ZHOU XZ, ZHANG BS. Study on cultivation techniques of high temperature selenium-enriched Pleurotus ostreatus[J]. Shandong Agricultural Sciences, 2013, 45(11): 63-65(in Chinese).
    [11] KIM YH, LEE HS, KWON HJ, PATNAIK BB, NAM KW, HAN YS, BANG IS, HAN MD. Effects of different selenium levels on growth and regulation of laccase and versatile peroxidase in white-rot fungus, Pleurotus eryngii[J]. World Journal of Microbiology and Biotechnology, 2014, 30(7): 2101-2109.
    [12] 刘壁容. 平菇液体发酵富硒菌丝体培养研究[D]. 长沙: 湖南农业大学硕士学位论文, 2005. LIU BR. Research on the seleium-rich mycelium of Pleurotus ostreatus by liquid fermentation[D]. Changsha: Master’s Thesis of Hunan Agricultural University, 2005(in Chinese).
    [13] LI XL, YAN LJ, LI Q, TAN H, ZHOU J, MIAO RY, YE L, PENG WH, ZHANG XP, TAN W, ZHANG B. Transcriptional profiling of Auricularia cornea in selenium accumulation[J]. Scientific Reports, 2019, 9: 5641.
    [14] WANG L, LI XY, WANG BB. Synthesis, characterization and antioxidant activity of selenium modified polysaccharides from Hohenbuehelia serotina[J]. International Journal of Biological Macromolecules, 2018, 120: 1362-1368.
    [15] 尚德静. 灵芝有机硒的生物转化及灵芝硒多糖的硒活性结构分析、抑癌作用的研究[D]. 大连: 大连理工大学博士学位论文, 2000. SHANG DJ. Biotransformation of selenium and Se activity structure of polysaccharide containing selenium in Ganoderma Lucidum [D]. Dalian: Doctoral Dissertation of Dalian University of Technology, 2000(in Chinese).
    [16] ZHOU F, YANG WX, WANG MK, MIAO YX, CUI ZW, LI Z, LIANG DL. Effects of selenium application on Se content and speciation in Lentinula edodes[J]. Food Chemistry, 2018, 265: 182-188.
    [17] 龚曦珊, 陈青君, 张国庆, 张佳艳, 赵亚京, 张娇, 赵敏睿. 硒对大球盖菇菌丝生长、子实体农艺性状和营养品质的影响[J]. 微生物学通报, 2023, 50(10): 4522-4532. GONG XS, CHEN QJ, ZHANG GQ, ZHANG JY, ZHAO YJ, ZHANG J, ZHAO MR. Selenium affects the mycelial growth, agronomic traits, and nutritional quality of Stropharia rugosoannulata[J]. Microbiology China, 2023, 50(10): 4522-4532(in Chinese).
    [18] 李红英, 陈菲菲, 孙举志, 殷红清, 问小龙, 向极钎. 硒对香菇品质的影响[J]. 北方园艺, 2022, 12: 106-113. LI HY, CHEN FF, SUN JZ, YING HQ, WEN XL, XIANG JQ. Effects of selenium on Lentinus edodesquality[J]. Northern Horticulture, 2022, 12: 106-113(in Chinese).
    [19] HU T, HUI GF, LI HF, G YB. Selenium biofortification in Hericium erinaceus (Lion’s Mane mushroom) and its in vitro bioaccessibility[J]. Food Chemistry, 2020, 331: 127287.
    [20] MILETIĆ D, TURŁO J, PODSADNI P, PANTIĆ M, NEDOVIĆ V, LEVIĆ S, NIKŠIĆ M. Selenium-enriched Coriolus versicolor mushroom biomass: potential novel food supplement with improved selenium bioavailability[J]. Journal of the Science of Food and Agriculture, 2019, 99(11): 5122-5130.
    [21] LI J, WEN S, ZHANG BY, WANG FH. Selenium enrichment of the edible medicinal mushroom Antrodia camphorata by submerged fermentation[J]. Molecules (Basel, Switzerland), 2023, 28(7): 3036.
    [22] 王明洋. 富硒杏鲍菇品质特性及其硒的化学形态研究[D]. 南京: 南京农业大学硕士学位论文, 2017. WANG MY. Study on quality characteristics of selenium-enriched Pleurotus eryngii and chemical speciation of selenium[D]. Nanjing: Master’s Thesis of Nanjing Agricultural University, 2017(in Chinese).
    [23] 周菲. 食用菌对外源硒的吸收转化及含硒菌糠在生物强化中的再利用研究[D]. 杨凌: 西北农林科技大学博士学位论文, 2022. ZHOU F. Study on absorption and transformation of exogenous selenium by edible fungi and reuse of selenium-containing mushroom bran in biological enhancement[D]. Yangling: Doctoral Dissertation of Northwest A&F University, 2022(in Chinese).
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

SUN Juzhi, LI Hongying, CHEN Feifei, QIN Daji, WEN Xiaolong, ZOU Yingchun. Screening of a selenium-enriching strain of Pleurotus ostreatus by ultraviolet mutagenesis and its application in nutrient enrichment[J]. Microbiology China, 2024, 51(7): 2368-2380

Copy
Share
Article Metrics
  • Abstract:174
  • PDF: 489
  • HTML: 386
  • Cited by: 0
History
  • Received:October 07,2023
  • Adopted:December 07,2023
  • Online: July 20,2024
  • Published: July 20,2024
Article QR Code