Four new records of botryosphaerialean fungi from Xanthoceras sorbifolium

Abstract:

[Background] Fungi of Botryosphaeriaceae are mostly major pathogens in agriculture and forestry. [Objective] To investigate the microfungi of Ascomycota from Xanthoceras sorbifolium in Changchun and Siping of Jilin province. [Methods] The specimens of microfungi were collected, and their morphology was observed and described in detail. After DNA extraction, the internal transcribed spacer (ITS) rDNA, ribosome large subunit (LSU), and translation elongation factor 1-alpha (tef1-α) of each species were sequenced. Phylogenetic trees were constructed based on maximum likelihood method, maximum parsimony method, and Bayesian inference. [Results] Four species of Botryosphaeriaceae were identified as Botryosphaeria sinensis, Phaeobotryon aplosporum, Phaeobotryon negundinis, and Phaeobotryon rhois, respectively. These four species of ascomycetes were reported on Xanthoceras sorbifolium for the first time. This paper described and illustrated the macroscopic, microscopic, and colony morphological characteristics of the above four new record species in detail. [Conclusion] P. negundinis was identified as a new record in China. B. sinensis, P. aplosporum, and P. rhois were identified as new records in Jilin.